In Vitro Organ Culture of the Bovine Intervertebral Disc
نویسندگان
چکیده
منابع مشابه
Correction: Loading-Induced Heat-Shock Response in Bovine Intervertebral Disc Organ Culture
[This corrects the article DOI: 10.1371/journal.pone.0161615.].
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Mechanical loading has been shown to affect cell viability and matrix maintenance in the intervertebral disc (IVD) but there is no investigation on how cells survive mechanical stress and whether the IVD cells perceive mechanical loading as stress and respond by expression of heat shock proteins. This study investigates the stress response in the IVD in response to compressive loading. Bovine c...
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INTRODUCTION: In vitro organ culture studies of the intervertebral disc (IVD) have been commonly used to understand the biologic and mechanical pathways contributing to disc degeneration. In particular, small animal models such as rat and rabbit have many advantages over large animals in an organ culture standpoint. Temporal and spatial stability of cell viability and extracellular matrix conte...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Development of a whole disc organ culture system to study human intervertebral disc
STUDY TYPE Basic science Objective: Low back pain is one of the most common health problems1 and is strongly associated with intervertebral disc degeneration, (IVD). Current treatments remove the symptoms without reversing or even retarding the underlying problem. Development of new therapy for the regeneration of the degenerative IVD is complicated by the lack of a validated long-term organ ...
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ژورنال
عنوان ژورنال: Spine
سال: 2006
ISSN: 0362-2436
DOI: 10.1097/01.brs.0000201302.59050.72